Genetic architecture of artemisinin-resistant Plasmodium falciparum

Laboratory Detection of Artemisinin-Resistant Plasmodium falciparum

Conventional 48-h in vitro susceptibility tests have low sensitivity in identifying artemisinin-resistant Plasmodium falciparum, defined phenotypically by low in vivo parasite clearance rates. We hypothesized originally that this discrepancy was explained by a loss of ring-stage susceptibility and so developed a simple field-adapted 24-h trophozoite maturation inhibition (TMI) assay focusing on...

Fitness of artemisinin-resistant Plasmodium falciparum in vitro.

OBJECTIVES Drug resistance confers a fitness advantage to parasites exposed to frequent drug pressure, yet these mutations also may incur a fitness cost. We assessed fitness advantages and costs of artemisinin resistance in Plasmodium falciparum in vitro to understand how drug resistance will spread and evolve in a competitive environment. METHODS Genotyping of SNPs, drug susceptibility assay...

Artemisinin-Resistant Plasmodium falciparum K13 Mutant Alleles, Thailand–Myanmar Border

Area and Costa Rica in general, free-ranging dogs are common , including at playgrounds and school yards, sites also vulnerable to nocturnal visits by raccoons. Dogs can have patent B. procyonis parasite infections and can play a role in transmission of the parasite from raccoons to humans. In Costa Rica, cases of larva migrans have been reported. The Unidad de Investigación y Análisis, Regis-t...

Artemisinin resistance in Plasmodium falciparum malaria.

BACKGROUND Artemisinin-based combination therapies are the recommended first-line treatments of falciparum malaria in all countries with endemic disease. There are recent concerns that the efficacy of such therapies has declined on the Thai-Cambodian border, historically a site of emerging antimalarial-drug resistance. METHODS In two open-label, randomized trials, we compared the efficacies o...

Artemisinin Resistance in Plasmodium falciparum Malaria